Although there are two different ways to perform restriction enzyme analysis with MacVector, there are also additional places where restriction enzyme sites are shown. All these tools use the same set of restriction enzyme files to recognise enzymes. These files are updated regularly from the REBASE database. The restriction enzymes are divided into multiple files. […]
The Map tab is very flexible and allows you to do almost all of the sequence manipulations you might need, without switching tabs. In addition to selecting segments of a sequence by clicking on Restriction Enzyme sites or Features, you can also directly select sequence even when the residues are not visible and represented by […]
We’ve previously discussed how every ligation is documented. You get a Frag annotation that contains the date, source sequence, enzymes used and any end modification that was done to that fragment during the ligation. However, we had regular requests to make it easier for users to document their constructs in other ways. For example, being […]
Although we think that the default appearance of sequence maps in MacVector is very pretty, sometimes the defaults are not to everybody’s taste! If you think this way, then changing how maps look is very easy. As usual with MacVector, there are many ways to quickly edit the appearance of a feature or multiple features. […]
MacVector understands the difference between circular DNA molecules and linear DNA molecules. Not just from the standpoint of how the molecule is displayed on the screen, but also in regard to how the analysis algorithms handle the junction at the ends of the circle. The Topology button controls the underlying linear versus circular state of […]
MacVector tries to optimize the Map graphics layout using a trade-off between performance and minimizing unnecessary white space. Sometimes the default settings we have chosen are not ideal, particularly if you are looking at the Map tab of an Align To Reference window where you have a large number of reads overlapping the same region. […]
You can use MacVector to create beautiful graphical representations of sequences with control over colors, patterns, fonts, symbols and many other aspects of the layout. The easiest way to get the graphics into another application is to simply choose Edit | Copy, switch to the target application and then choose Edit | Paste. The graphics […]
Many MacVector users do not realize that you can view sequences at the residue level in the Map tab as well as the Editor tab. This has the advantage that you can see restriction enzyme cut sites (including staggered sticky ends) along with the graphical display of features aligned to the residues. You can even […]
The Map tab of MacVector is a powerful way to visualise and interact with your sequences. All analysis tools will work directly in the Map tab. You can design primers, ligate and digest fragments from the Cloning Clipboard, visualise translated CDS regions and much more. In fact one of the only tasks, you will need […]
OK, so this is a very simple tip that many users already know about. But we get enough queries about this functionality that its useful to go over it. The Editor tab has a fair amount of functionality, but it really is designed for editing of sequences at the residue level – we don’t directly […]