MacVectorTip: Quality scoring of manual edits to your contigs.

Quality scoring of Assemblies and Align to Reference alignments can be visualized directly on the sequence. Residues can be shaded according to their quality scores. These can be displayed anywhere quality values are available, including de novo and reference assemblies in Assembler and Align to Reference alignments. The intensity of the shading of residues indicates […]

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How to design a digest to screen minipreps after a ligation.

MacVector’s Agarose Gel tool can be used to quickly design a restriction digest to screen minipreps following a ligation.  (View full size on website…) Replicate your ligation in MacVector. Select the two sites, for subcloning your targeted gene, and click DIGEST. Drag the digested fragment from the Cloning Clipboard to your vector click LIGATE. Create […]

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