General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

Tag Archives: codon optimization

How to find one-out Restriction Sites that will change a protein coding sequence

If you are interested in making changes to a protein sequence, it is often useful to make a change to the coding DNA that will create a restriction enzyme site. Conversely, there may be times that you would like to create a site without affecting a protein coding region. You can accomplish this using the […]

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Optimizing the Reverse Translation function

The Analyze | Reverse Translation menu option lets you create a DNA sequence from a Protein sequence, reverse translated using a specific Genetic Code (by default, the Universal Genetic Code). The default option creates a DNA sequence with N’s and other ambiguities reflecting the degeneracy of the genetic code. This is great if you want […]

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