There can be times when you are messing about with open reading frames, inserting residues to change frames to try to get the perfect CDS fusion. The MacVector single sequence Editor will show those (click and hold on the “Display” toolbar button) but if you select and copy, only the DNA sequence (with any overlapping […]
The Analyze | Reverse Translation menu option lets you create a DNA sequence from a Protein sequence, reverse translated using a specific Genetic Code (by default, the Universal Genetic Code). The default option creates a DNA sequence with N’s and other ambiguities reflecting the degeneracy of the genetic code. This is great if you want […]
MacVector 15.5 automatically scans every DNA sequence window for open reading frames and displays the results in the Map tab. The setting for this are controlled by the MacVector | Preferences | DNA Map pane, along with the automatic Show restriction sites settings. The Minimum Number of Codons setting is fairly obvious. 5’ ends are […]
MacVector displays amino acid translations in many different result windows. You can drill down to the residue level in the Map tab and see translations of CDS and other translatable features and see translations in the plain text views and the Quicktest Primer interface. The translations can be viewed as either single letter codes or […]
The Analyze | Reverse Translation menu option lets you create a DNA sequence from a Protein sequence, reverse translated using a specific Genetic Code (by default, the Universal Genetic Code). The default option creates a DNA sequence with N’s and other ambiguities reflecting the degeneracy of the genetic code. This is great if you want […]
Where appropriate, features can be shown as residues when there is sufficient space to show them (for example when zoomed to residue). By default this is enabled for certain features, e.g. CDS features, genes, but it is controlled from the Symbol Editor and can be turned on/off for most features. In the Map tab, double […]
MacVector displays amino acid translations in many different result windows. You can drill down to the residue level in the Map tab and see translations of CDS and other translatable features and see translations in the plain text views and the Quicktest Primer interface. The translations can be viewed as either single letter codes or […]
For those of you who like to type in DNA sequences, or do manual editing to the sequences, it’s really useful to be able to see the translations above or below the sequence residues in the Editor tab of the single sequence window. If you are a long term user, you’ll be familiar with the […]
Following on from my last post about turning on translations in the sequence Editor, I thought I should point out how to display amino acid translations using the 3-letter code rather than the default single-letter code. I don’t know about you, but I have a hard time remembering that “W” is the single-letter IUPAC code […]
If you have used MacVector for many years, you’ll remember the days when the single sequence editor was THE main way you interacted with MacVector. When MacVector 10 was introduced back in 2007, the Editor became just one tab in the single sequence window along with Map, Features and Annotations tabs, all of which at […]